These "QX3" images make use of the applet version of an application called ImageJ. This version should shortly be running in a separate "ImageJ" window. The download will take a few seconds with an Ethernet connection or about two minutes using a 28.8 modem. Quit ImageJ by clicking on the "ImageJ" window's close button. When you go to the Windows version of one of the exercises below the ImageJ application will remain running in the background.

(NOTE: ImageJ opens up without any problems using either Netscape of Internet Explorer on Windows. Internet Explorer 4.5 and above works fine on the Macintosh, but there is no Netscape support at this time on the Macintosh. If you wish to download a version of ImageJ to your computer's hard drive so you can evaluate your own images a download can be obtained at the following site.

When you view an image with ImageJ you will have three windows open at a time (the WEB page, ImageJ and at least one image) so you may want to go to your control panel on your computer and change the monitor size so that you can readily move between the different windows.

Measuring using ImageJ

Go to File, Open Samples and open your image. This is a view that was photographed with the QX3. Now go to Pugins and select Microscope Scale In the box that comes up select the magnification of your image and click Add Scale Bar - and then OK. The next window asks you what color you want the scale bar to be and its location on the image. Make any changes and click OK. Having a micron (µm or micrometer) bar on an image makes it possible to calibrate the image. Follow the directions below to calibrate your image and to measure anything on it. (If you want to save your calibrated image - Save as a Tiff file.)

Use your Magnifying Tool and click on the scale bar. This will enlarge your view. (To go back hold down the Option key and click) Take your Straight Line Tool and click on the left side of this scale and draw the line to the right side. Go up to Analyze, and click on Measure. Now go to Analyze, Set Measurements and the only box selected should be perimeter. Click OK and then go to Analyze, Set Scale. (You will probably have a reading of so many pixels) Enter whatever number is below your micron bar for Known Distance. Put µm In the Unit of Length box - if it is not already entered. Click OK

To measure one of your own images go to File, Open and select your image. Calibrate it and perform your measurements.

LIGHT MICROSCOPE

Ruler under 10X	Ruler under 60X	Ruler under 200X

Last updated:September 20, 2003

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