FlBeetle

Show ImageJ application.

Part A: Pixelating

Go to File, Open Samples and open image Flour Beetle. Flour beetles are used in a number of classroom activities that range from studying the life cycle of this insect to complex genetics experiments. At the top of the image you will see the following information.

This means the Flour Beetle image is 517 pixels wide and 426 pixels high.

In the image below some of the pixels from the above photograph have been enlarged with the Magnifying Tool (To go back hold down the Option key and click with the magnifier) and then copied to the right. Your task is to figure out where on the left side of the image the right side came from? The X and Y coordinates of one pixel are given and should help you find the area that will match the right side of the image.

Part B:Colorizing

Reduce the image back to its regular size. Go up to Image, Color Tables, Fire and your image should appear similar to what is shown on the right. "Colorizing" can sometimes be helpful to better see certain details. Try the other Color Tables and see which one you prefer.

Part C:Measuring

Go up to File and select Revert. If the image does not revert back to "normal", then close it and reopen the image. At the bottom of the image you will see the scale that is shown below.

The .085kx means that .085 is multiplied by 1000 for a magnification of 85X. The white band is a scale that is equal to 120 micrometers (µ) or 0.120 millimeters. To measure anything on this image it is necessary to calibrate it.

Take your Straight Line Tool and click on the left side of the white band and draw the line to the right side of the band. Go up to Analyze, and click on Measure. Now go to Analyze, Set Measurements and the only box selected should be perimeter. Click OK and then go to Analyze, Set Scale. (You will probably have a reading of around 50 pixels) Enter 120 for Known Distance and then put micrometers in the Unit of Measurement box. Click OK (If you have further questions about this you can go to Open Samples and open the file Cal1)

Now take your Straight Line Tool and draw a line between the two eyes of the Flour Beetle. Go up to Analyze, and click on Measure. You should have the length showing in the box under ImageJ. Record your results in the results table.

Now take your Straight Line Tool and draw a line between from the left side of the image to the right. Go up to Analyze, and click on Measure and record your answer in the results table.

Now take your Magnifying Tool and enlarge anywhere on the image as far as much as you can. Measure the length of 1 pixel. If this image is 517 pixels wide how does this measurement compare to what you have for the width of the image in µ?

Reference

The follow are WEB reference sites for information on Flour Beetles.

The Red Flour Beetle and the Confused (that's right confused) Flour Beetle.

One of the all time great sites for Flour Beetle images.

Results

(You can also Go to File, Open Samples and Open the image Results which is a copy of this Results section. While this page cannot be printed out - at this time - it can be saved as a JPEG file to your desktop. You can then open it with any graphics program,type out the answers and then print it out from your program.

You can also enter the results on this image using the Text Tool. To do this first go to Edit, Options, Fonts and select something like Geneva - 12 pt - Bold. Then click on the Results Image in the answer space and draw out the yellow rectangle that appears and start typing. When you are finished go to Edit and Select Draw. What you have typed should now be present.)

1. Where did the pixel image in Part A come from?

2. What is the distance between the eyes of the Flour Beetle?

Table 1:

Image Width (µ)	Pixel Width (µ)	Pixel Width Xs # of pixels in image width

3. How does this last measurement compare to what you have for the width of the image in µ?